article Open AccessTop 1% cited
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Nature Biotechnology · 2014 · Vol. 33(2) · pp. 187–197
Shengdar Q. Tsai✉(Harvard University)Zongli Zheng(Harvard University)Nathalie T. Nguyen(Massachusetts General Hospital)Matthew Liebers(Massachusetts General Hospital)Ved V. Topkar(Massachusetts General Hospital)Vishal Thapar(Massachusetts General Hospital)Nicolas Wyvekens(Massachusetts General Hospital)Cyd Khayter(Massachusetts General Hospital)A. John Iafrate(Harvard University)Long P. Le(Harvard University)Martin J. Aryee(Harvard University)J. Keith Joung(Harvard University)
CRISPR and Genetic EngineeringRNA regulation and diseaseAdvanced biosensing and bioanalysis techniquesCRISPRComputational biologyGenomeBiologyChromatinGenome editingDNA sequencingGuide RNAGenomicsDNA
MeSH terms
RNA, Guide, CRISPR-Cas SystemsCell LineHumansOligodeoxyribonucleotidesGenome, HumanRNA EditingRNA, Guide, KinetoplastidaDNA Breaks, Double-StrandedHigh-Throughput Nucleotide SequencingCRISPR-Cas Systems
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39
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References
Genome engineering using the CRISPR-Cas9 system
Nature Protocols · 2013 · 11,552 citations
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Nature Biotechnology · 2013 · 2,476 citations
DNA targeting specificity of RNA-guided Cas9 nucleases
Nature Biotechnology · 2013 · 4,831 citations
CRISPR-Cas systems for editing, regulating and targeting genomes
Nature Biotechnology · 2014 · 3,157 citations
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Science · 2012 · 16,935 citations
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
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Development and Applications of CRISPR-Cas9 for Genome Engineering
Cell · 2014 · 5,789 citations
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