articleTop 1% cited
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Nature Biotechnology · 2016 · Vol. 34(2) · pp. 184–191
John G. Doench✉(Broad Institute)Nicolò Fusi(Microsoft (United States))Meagan E. Sullender(Broad Institute)Mudra Hegde(Broad Institute)Emma W Vaimberg(Broad Institute)Katherine Donovan(Broad Institute)Ian C. P. Smith(Broad Institute)Zuzana Tóthová(Broad Institute)Craig B. Wilen(Washington University in St. Louis)Robert C. Orchard(Washington University in St. Louis)Herbert W. Virgin(Washington University in St. Louis)Jennifer Listgarten(Microsoft (United States))David E. Root(Broad Institute)
CRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsCRISPRCas9Subgenomic mRNAGenome editingGuide RNAComputer scienceComputational biologyGenome engineeringGenomeMetric (unit)
MeSH terms
RNA, Guide, CRISPR-Cas SystemsAnimalsDrug ResistanceGenetic EngineeringHumansGene LibraryGenomeRNA, Guide, KinetoplastidaGenomicsCell Line, TumorMiceCRISPR-Cas Systems
Citations
4,855
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References
54
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References
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Proceedings of the National Academy of Sciences · 2003 · 10,009 citations
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Science · 2013 · 9,293 citations
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Nature Biotechnology · 2014 · 2,254 citations
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Bioinformatics · 2011 · 7,557 citations
Improved vectors and genome-wide libraries for CRISPR screening
Nature Methods · 2014 · 5,428 citations
DNA targeting specificity of RNA-guided Cas9 nucleases
Nature Biotechnology · 2013 · 4,831 citations
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Nature · 2012 · 8,404 citations
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Science · 2012 · 16,935 citations
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