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Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Genome biology · 2016 · Vol. 17(1) · pp. 148–148

Maximilian Haeussler✉(University of California, Santa Cruz)Kai Schönig(Heidelberg University)Hélène Eckert(Centre National de la Recherche Scientifique)Alexis Eschstruth(Centre National de la Recherche Scientifique)Joffrey Mianné(Mary Lyon Centre at MRC Harwell)Jean-Baptiste Renaud(Université Paris-Sud)Sylvie Schneider‐Maunoury(Centre National de la Recherche Scientifique)Alena Shkumatava(Centre National de la Recherche Scientifique)Lydia Teboul(Mary Lyon Centre at MRC Harwell)Jim Kent(University of California, Santa Cruz)Jean‐Stéphane Joly(Université Paris-Sud)Jean‐Paul Concordet(Centre National de la Recherche Scientifique)

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Abstract

To make these guidelines easily accessible to anyone planning a CRISPR genome editing experiment, we built a new website ( http://crispor.org ) that predicts off-targets and helps select and clone efficient guide sequences for more than 120 genomes using different Cas9 proteins and the eight efficiency scoring systems evaluated here.

CRISPR and Genetic Engineering RNA and protein synthesis mechanisms RNA regulation and diseaseBiologyCRISPRPopularitySelection (genetic algorithm)Human geneticsComputational biologyRNACas9Genome BiologyGuide RNA

MeSH terms

Gene EditingRNA, Guide, CRISPR-Cas SystemsAlgorithmsPromoter Regions, GeneticRNA, Small NuclearSoftwareGenomeRNA, Guide, KinetoplastidaInternetCRISPR-Cas Systems