article Open AccessTop 1% cited
Genome engineering using the CRISPR-Cas9 system
Nature Protocols · 2013 · Vol. 8(11) · pp. 2281–2308
F. Ann Ran✉(Harvard University)Patrick D. Hsu(Massachusetts Institute of Technology)Jason Wright(Massachusetts Institute of Technology)Vineeta Agarwala(Massachusetts Institute of Technology)David Scott(Broad Institute)Feng Zhang(Massachusetts Institute of Technology)
CRISPR and Genetic EngineeringRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesCRISPRGenome engineeringCas9Genome editingGuide RNABiologyComputational biologyNucleaseGenomeHomology directed repair
MeSH terms
Base SequenceCell LineDeoxyribonucleasesDNA Mutational AnalysisDNA RepairGenetic EngineeringHumansMolecular Sequence DataTransfectionMutagenesisGenomeCell Culture TechniquesHEK293 CellsDNA End-Joining RepairGenotyping Techniques
Funding
- National Science Foundation
- Damon Runyon Cancer Research Foundation
- Fund for Astrophysical Research
- McKnight Foundation
- National Institutes of Health
Citations
11,552
FWCI
149.35
field-weighted impact
References
76
Percentile
100%
vs. same field & year
Citations per year
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References
Efficient genome editing in zebrafish using a CRISPR-Cas system
Nature Biotechnology · 2013 · 2,984 citations
Genome editing with engineered zinc finger nucleases
Nature Reviews Genetics · 2010 · 2,248 citations
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Nature Biotechnology · 2013 · 2,476 citations
RNA-Guided Human Genome Engineering via Cas9
Science · 2013 · 9,293 citations
DNA targeting specificity of RNA-guided Cas9 nucleases
Nature Biotechnology · 2013 · 4,831 citations
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Science · 2012 · 16,935 citations
Multiplex Genome Engineering Using CRISPR/Cas Systems
Science · 2013 · 15,534 citations
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
Nature Biotechnology · 2013 · 3,285 citations
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