Influence of sodium nitroprusside on sperm mitochondrial membrane potential of frozen thawed buffalo semen

Abstract

The present investigation was conducted to study the effect of exogenous addition of sodium nitroprusside (SNP), a nitric oxide (NO) donor on sperm mitochondrial membrane potential (MMP) of frozen thawed buffalo semen. Frozen semen straws from 6 buffalo bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-51. The straws were thawed at 37 ˚C for 30 seconds and emptied into 15 ml sterile plastic centrifuge tube containing 1 ml of capacitation medium (control), addition of 100 µM SNP to capacitation medium (SNP treatment I) and 100 nM SNP to capacitation medium (SNP treatment II) and incubated at 37 ˚C for 1 hour. After incubation, sperm MMP was determined by JC-1(5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide) stain technique. Significantly (P