Acrosome integrity and mitochondrial membrane potential of frozen thawed buffalo semen treated with heparin binding protein

Abstract

The experiment was conducted to study the sperm acrosome integrity and mitochondrial membrane potential (MMP) of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37 ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25µg/mL (treatment I), 50µg/mL (treatment II) and 100µg/mL (treatment III) of HBP. The contents were incubated at 37 ºC for 2 hours. After incubation, sperm acrosomal integrity was assessed by Giemsa stain method. In control, HBP treatment I, II and III, 51.50% ± 1.29, 46.30% ±0.86, 43.40% ± 0.66and 42.15% ± 0.40 spermatozoa, respectively had intact acrosome. But, 48.50% ±1.29, 53.70% ± 0.86, 56.60% ± 0.66and 57.85% ± 0.40 spermatozoa in control, HBP treatment I, II and III respectively had lost acrosome. Significantly (P