Osteopontin ameliorates sodium nitroprusside induced free radical damage on sperm motility of frozen thawed buffalo semen

Abstract

The experiment was undertaken to study sodium nitroprusside (SNP) induced free radical damage on sperm motility of frozen thawed buffalo semen and the ameliorative effects of osteopontin (OPN) supplementation on sperm motility. Buffalo frozen semen straws from 8 ejaculates of 6 bulls were procured from Central Frozen Semen Production and Training Institute, Hessarghatta, Banglore and stored at Semen Bank, Madras Veterinary College, Chennai. The semen straws were thawed and seminal plasma and semen extender were removed from spermatozoa by centrifugation. Spermatozoa were suspended in 1mL capacitation medium (control), with the addition of 100µg/mL of OPN (treatment I) or 100µM/mL of SNP (treatment II) or 100µg/mL of OPN + 100µM/mL of SNP (treatment III). Semen with capacitation medium alone without any supplementation served as control. The contents were incubated at 37ºC for 4 h and the post capacitation sperm motility was observed under bright field microscopy. The post capacitation sperm motility was significantly (P