Lipid peroxidation status and plasma membrane integrity of frozen thawed buffalo semen treated with heparin binding protein

Abstract

The experiment was conducted to study the sperm lipid peroxidation status and plasma membrane integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37 oC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25µg/mL (treatment I), 50µg/mL (treatment II) and 100µg/mL (treatment III) of HBP. The contents were incubated at 37 oC for 2 hours. The sperm LPO status was measured by production of malondialdehyde (MDA) level. The MDA levels were significantly (P