Effect of sodium nitroprusside on sperm lipid peroxidation status of frozen thawed buffalo semen

Abstract

The experiment was conducted to assess the effect of exogenous supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor on lipid peroxidation (LPO) status of frozen thawed buffalo semen. Frozen semen straws from 6 buffalo bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-51. The straws were thawed at 37 ˚C for 30 seconds and emptied into 15 ml sterile plastic centrifuge tube containing 1 ml of capacitation medium (control), addition of 100 µM SNP to capacitation medium (SNP treatment I) and 100 nM SNP to capacitation medium (SNP treatment II) and incubated at 37 ˚C for 1 hour. After incubation, sperm LPO status was measured by production of malondialdehyde (MDA) level. The MDA levels were significantly (P