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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Clinical Chemistry · 2009 · Vol. 55(4) · pp. 611–622
Stephen A. Bustin✉(Queen Mary University of London)Vladimı́r BenešJeremy A. Garson(University College London Hospitals NHS Foundation Trust)Jan Hellemans(Ghent University Hospital)Jim F. Huggett(University College London)Mikael Kubista(Tataa Biocenter (Sweden))Reinhold Mueller(Sequenom (United States))Tania NolanMichael W. PfafflGregory L. Shipley(The University of Texas Health Science Center at Houston)Jo Vandesompele(Ghent University Hospital)Carl T. Wittwer(University of Utah)
Abstract
Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.
Molecular Biology Techniques and ApplicationsEnvironmental DNA in Biodiversity StudiesGene expression and cancer classificationReal-time polymerase chain reactionComputer scienceComputational biologyInformation retrievalStatisticsBiologyMathematicsGeneticsGene
MeSH terms
HumansTerminology as TopicNucleic AcidsPublishingTime FactorsPolymerase Chain ReactionMolecular Diagnostic TechniquesReverse Transcription
Funding
- Bowel and Cancer Research
- ARUP Institute for Clinical and Experimental Pathology
Citations
15,780
FWCI
166.99
field-weighted impact
References
92
Percentile
100%
vs. same field & year
Citations per year
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References
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Nature Genetics · 2001 · 4,089 citations
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Genome biology · 2002 · 19,887 citations
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
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Quantification of mRNA using real-time RT-PCR
Nature Protocols · 2006 · 2,241 citations
Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia – A Europe Against Cancer Program
Leukemia · 2003 · 1,520 citations
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Nucleic Acids Research · 2002 · 7,348 citations
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
Cancer Research · 2004 · 7,130 citations
A new mathematical model for relative quantification in real-time RT-PCR
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