article Open AccessTop 1% cited
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Genome biology · 2002 · Vol. 3(7) · pp. RESEARCH0034–RESEARCH0034
Jo Vandesompele✉(Ghent University Hospital)Katleen De Preter(Ghent University Hospital)Filip Pattyn(Ghent University Hospital)Bruce Poppe(Ghent University Hospital)Nadine Van Roy(Ghent University Hospital)Anne De Paepe(Ghent University Hospital)Frank Speleman(Ghent University Hospital)
Abstract
The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which, among other things, opens up the possibility of studying the biological relevance of small expression differences.
Molecular Biology Techniques and ApplicationsRNA Research and SplicingGene expression and cancer classificationHousekeeping geneNormalization (sociology)BiologyDatabase normalizationComputational biologyGeneGene expression profilingGene expressionMicroarray analysis techniquesGenetics
MeSH terms
AlgorithmsFemaleHumansReference StandardsRNATime FactorsTumor Cells, CulturedReproducibility of ResultsDNA, ComplementaryReverse Transcriptase Polymerase Chain ReactionGene Expression Profiling
Funding
- Fonds Wetenschappelijk Onderzoek
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19,887
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