articleTop 1% cited
Quantification of mRNA using real-time RT-PCR
Nature Protocols · 2006 · Vol. 1(3) · pp. 1559–1582
Tania Nolan✉Rebecca Hands(Queen Mary University of London)Stephen A. Bustin(Queen Mary University of London)
Molecular Biology Techniques and ApplicationsRNA Research and SplicingRNA and protein synthesis mechanismsComputational biologySnapshot (computer storage)Database normalizationReal-time polymerase chain reactionBiologyMessenger RNAReverse transcription polymerase chain reactionStandardizationNormalization (sociology)Computer science
MeSH terms
Data Interpretation, StatisticalRNA, MessengerReverse Transcriptase Polymerase Chain ReactionGene Expression Profiling
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References
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Genome biology · 2002 · 19,887 citations
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
Nature Biotechnology · 1993 · 2,161 citations
IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin
Journal of Clinical Investigation · 2004 · 2,225 citations
A novel method for real time quantitative RT-PCR.
Genome Research · 1996 · 2,093 citations
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
Neuroscience Letters · 2003 · 3,722 citations
IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin
Journal of Clinical Investigation · 2004 · 2,111 citations
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