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Analyzing real-time PCR data by the comparative CT method

Nature Protocols · 2008 · Vol. 3(6) · pp. 1101–1108
Thomas D. SchmittgenKenneth J. Livak
Molecular Biology Techniques and ApplicationsRNA Research and SplicingGene expression and cancer classificationGene expressionGeneComputational biologyReal-time polymerase chain reactionBiologyComputer scienceGenetics

MeSH terms

AnimalsBreast NeoplasmsComputer SystemsData Interpretation, StatisticalFemaleHumansPancreasPancreatic NeoplasmsRNARNA, MessengerTranscription FactorsGene ExpressionPolymerase Chain ReactionHomeodomain ProteinsGenes, bcl-2
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References
A new mathematical model for relative quantification in real-time RT-PCR
Nucleic Acids Research · 2001 · 34,549 citations
Cluster analysis and display of genome-wide expression patterns
Proceedings of the National Academy of Sciences · 1998 · 16,353 citations
Real-time quantification of microRNAs by stem-loop RT-PCR
Nucleic Acids Research · 2005 · 4,966 citations
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