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Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

Science · 1995 · Vol. 270(5235) · pp. 467–470

Mark Schena✉(Stanford University)Dari Shalon✉(Stanford University)Ronald W. Davis✉(Stanford University)Patrick O. Brown✉(Stanford University)

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Abstract

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

Gene expression and cancer classification Chromosomal and Genetic Variations Plant Virus Research StudiesDNA microarrayGeneGene expressionMicroarrayDNABiologyMessenger RNAMolecular biologyArabidopsisNucleic acid thermodynamics

MeSH terms

DNA-Binding ProteinsGenetic TechniquesHumansMolecular Sequence DataNucleic Acid HybridizationRNA, MessengerTranscription FactorsRNA ProbesGene ExpressionGenome, HumanPolymerase Chain ReactionGenes, PlantArabidopsisDNA, ComplementaryHomeodomain Proteins

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Cluster analysis and display of genome-wide expression patterns