article Open AccessTop 10% cited
A standard curve based method for relative real time PCR data processing
BMC Bioinformatics · 2005 · Vol. 6(1) · pp. 62–62
Alexey A. Larionov✉(Western General Hospital)Andreas Krause(Novartis (Switzerland))William R. Miller(University of Edinburgh)
Abstract
A standard curve based procedure for PCR data processing has been compiled and validated. It illustrates that standard curve design remains a reliable and simple alternative to the PCR-efficiency based calculations in relative real time PCR.
Molecular Biology Techniques and ApplicationsForensic and Genetic ResearchRNA Research and SplicingComputer scienceDNA microarrayStandard curveComputational biologyData miningBiologyGenetics
MeSH terms
Sensitivity and SpecificitySoftwareReproducibility of ResultsGene Expression Regulation, NeoplasticPolymerase Chain ReactionArtifactsDNA PrimersRNA, ComplementaryDNA, ComplementaryComputational BiologyReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingAlgorithmsBreast NeoplasmsCloning, Molecular
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References
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Genome biology · 2002 · 19,887 citations
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Nucleic Acids Research · 2002 · 7,348 citations
A new mathematical model for relative quantification in real-time RT-PCR
Nucleic Acids Research · 2001 · 34,549 citations
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