GC-MS analysis on the methanolic extract of Trichosanthes anguina L. root and leaves

Abstract

Phyto Chemical Screening of Trichosanthes anguina L. (snake gourd) root and leaves were done by GC-MS analysis for the identification of secondary metabolites. In this paper, the results of the analysis of methanolic extract analysed by GC-MS technique (Thermo GC-Trace Ultra Version 5.0, Fisher, USA) are presented. The 1.0 ml/min volume of methanolic extract sample was injected into the instrument and the compounds are identified and analyzed by splitless injection technique. The compounds were separated using Helium (1.0 ml/min) as the carrier gas. The GC-MS analysis for Trichosanthes anguina L. provides eight major peaks determining the presence of twelve major compounds namely ester (15.13%); alcohol (2.12 %); hetero cyclic compound (19.67%); amine/amide (0.51/0.41%); thioether (0.51%); diene (9.88%); aldehyde (9.88 %); nitro compound (1.35 %); ketone (0.56%); alkene (0.56 %); amino compound (1.17 %) and amino acid (0.61%).The presence of various main bioactive compounds namely furan, pyrrole-1-oxide, 4-cyanomethyl-3- pyrrolepropione, nitrilemethyl esters, hetero cyclic compounds which confirm the medicinal importance of the plant.