Assessment of bioactive compounds in Brassica juncea using chromatographic techniques

Abstract

The investigation was carried out to determine the phytochemical components from methanolic extract of Indian mustard using High performance liquid chromatography (HPLC) and gas-chromatography mass spectrometry (GC-MS). GC-MS analysis of the extract led to the identification of more than 5 compounds in all the genotypes together. The major compounds in these mustard genotypes were found to be methallyl cyanide; allyl isothiocyanate; Oxime-methoxy-phenyl; 1-butene-4-isothiocyanato; 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl; 2-Furancarboxaldehyde; 5-(hydroxym-ethyl)-methyl-beta-d-galactopyranoside. Moreover, the genotype also showed allyl isothiocyantes in small percentage. Fatty acid analysis of Indian mustard also showed the presence of good amount of oleic acid, linolic acid and palmitic acid. The detection of these compounds in seeds of mustard genotypes highlights the pharmaceutical properties of this crop. The results inferred that these genotypes contain variable patterns of bioactive compounds and could be used as natural antioxidant source for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic.