Comparatives study of antioxidant activity and total phenolic contents of pomegranate and orange peels extracts

Abstract

Antioxidants were extracted from the pomegranate and orange peels by ethanol and their activity were evaluated by DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging, Ferric-reducing antioxidant power assay. The total phenolic compound (TPC) was determined by the Folin-Ciocalteu method to quantify the amounts of Phenolic contents. The (Pomegranate Peel Extracts) PPE exhibited higher total phenolic content (TPC) 139.40 mg/g GAE while the orange peel extracts (OPE) showed three times lower TPC of 35.73 mg/g GAE. The result of antioxidants activity of PPE showed the radical scavenging activity of 92.38% at the concentration of 35µg/mL where as the OPE showed the radical scavenging activity 85.68 % at a higher concentration of 700 µg/mL. The results of FRAP of the PPE showed the Abs range of 0.08-1.40Abs at700nm while the results of the OPE exhibited 0.08-0.24 Abs at700nm. The PPE showed about six times higher reducing power compared to OPE, at the concentration of 700 μg/mL and exhibited the 1.40 Abs at 700 nm while at the same concentration OPE showed only 0.24 Abs at 700 nm.The PPE showed a higher reducing power of 1.43 optical densities. The above results indicate that the PPE is good source of the phenolic compounds and higher antioxidant activity as compared to OPE.