In-vitro antioxidant activity of freeze dried extracts fruit peel waste (Punica granatum and Citrus sinensis) and its effect in α-linoleic acid model system

Abstract

Every year huge quantities of fruit waste are generated by fruit Industries. These wastes are can be recover as natural antitoxins which would be beneficial to the society and individual. Antioxidants from fruit wastes have attracted the attention of researchers as they are cheaper in cost, easy to derive, and do not have any side effects. Thus the current investigation was focused on to extract natural antioxidant and evaluates their properties through various in vitro assays. The pomegranate peel exhibited higher total phenolic content (TPC) of 160.80 mg/g GAE while the orange peel (OPE) showed lower. Total flavonoid contents OPE showed the highest (2.955 mg/g quercetin equivalent), while the PPE showed 0.98mg/g quercetin equivalent. The DPPH radical scavenging activity exhibited a dose dependent activity. The PPE extract showed the radical scavenging activity of 94.25 at the concentration of 35µg/mL whereas the same at the same concentration BHA showed inhibition of 95.59% which was almost equal to BHA. The highest chelating activity was 28.42% for PPE recorded at a concentration of 1000 µg/mL, while at the same concentration the chelating activity of OPE was half of that of PPE. Lipid peroxidation inhibition activity of PPE and OPE was observed for 7 days under controlled temperature condition. The aqueous ethanolic extract of PPE was the strongest inhibitor compare to OPE.