Studies on qualitative, quantitative, phytochemical analysis and screening of in vitro biological activities of Leucas indica (L) VAR. Nagalapuramiana

Abstract

Leucas indica var. nagalapuramiana is a high value medicinal plant of Seshachalam hill range of Eastern Ghats, Andhra Pradesh. The present study was conducted to determine the yield of extract, qualitative, quantitative analysis, antimicrobial activity and antioxidant activity of the crude extracts of Leucas indica (L) var. nagalapuramiana. The extracts were prepared by using Hexane, Acetone, Chloroform and Methanol solvents. The yield of extract was calculated for all the four solvents and they are studied for qualitative analysis of phytochemical compounds. The plant extracts were positive for a wide range of bioactive compounds and further they are studied for total phenol and flavonoid contents. The extracts are subjected to test their biological activities by few in vitro tests like anti-bacterial activity, anti- fungal activity and anti- oxidant activity. The present study revealed the presence of alkaloids, phenols, flavonoids, steroids, tannins, saponins, and reducing sugars. Quantitative analysis of the total phenols was 105 µgGAE/µg and flavonoids was 62.34 µg Rutin/µg. Antimicrobial activity was found to be moderate and a better antioxidant activity of IC50 365.5 µg was noted in methanol extracts.