reviewTop 1% cited

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

Applied Microbiology and Biotechnology · 2003 · Vol. 60(5) · pp. 523–533

K. Terpe✉(Protein Express (United States))

Monoclonal and Polyclonal Antibodies Research Peptidase Inhibition and Analysis Protein purification and stabilityProtein tagMaltose-binding proteinFusion proteinRecombinant DNABiochemistryFLAG-tagTandem affinity purificationThioredoxinProteomicsMyc-tag

MeSH terms

Amino Acid SequenceBinding SitesCalmodulinCarrier ProteinsCelluloseGlutathione TransferaseHistidineIndustryOligopeptidesPeptide FragmentsPeptidesProtein BindingRecombinant Fusion ProteinsRecombinant ProteinsRibonucleases

Citations

1,329

FWCI

46.11

field-weighted impact

References

119

Percentile

100%

vs. same field & year

Citations per year

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References

A generic protein purification method for protein complex characterization and proteome exploration

Nature Biotechnology · 1999 · 2,700 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

Molecular and Cellular Biology · 1985 · 2,434 citations

Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

Analytical Biochemistry · 1991 · 1,761 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Gene · 1988 · 671 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Gene · 1988 · 5,965 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Journal of Chromatography A · 1987 · 1,109 citations

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

Applied Microbiology and Biotechnology · 2003 · 1,329 citations

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