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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Gene · 1988 · Vol. 67(1) · pp. 31–40
Donald B. Smith✉(The Royal Melbourne Hospital)Kevin S. Johnson(University of Melbourne)
Biochemical and Molecular ResearchInsect Resistance and GeneticsEnzyme Structure and FunctionBiologyFusion proteinEscherichia coliAffinity chromatographyGlutathione S-transferaseBiochemistryGlutathioneMolecular biologyExpression vectorRecombinant DNA
MeSH terms
Amino Acid SequenceAnimalsBase SequenceCloning, MolecularEscherichia coliGenesGenetic VectorsGlutathione TransferaseMolecular Sequence DataPlasmidsRecombinant Fusion ProteinsRecombinant ProteinsSchistosoma japonicum
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References
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Gene · 1984 · 712 citations
Determination of protein: A modification of the lowry method that gives a linear photometric response
Analytical Biochemistry · 1972 · 5,526 citations
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
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