articleTop 1% cited
Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
Analytical Biochemistry · 1991 · Vol. 192(2) · pp. 262–267
Kun‐Liang Guan✉(Walther Cancer Foundation)J.E. Dixon(Purdue University West Lafayette)
Cancer Research and TreatmentsBacteriophages and microbial interactionsProtein purification and stabilityFusion proteinBiochemistryAffinity chromatographyTobacco etch virusCleavage (geology)Recombinant DNATandem affinity purificationEscherichia coliThrombinBiology
MeSH terms
Amino Acid SequenceAnimalsBase SequenceChromatography, AffinityCloning, MolecularEscherichia coliGlutathione TransferaseGlycineMolecular Sequence DataPhosphoprotein PhosphatasesPlasmidsRecombinant Fusion ProteinsThrombinRestriction MappingGene Expression
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References
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Gene · 1988 · 5,965 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature · 1970 · 251,243 citations
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