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Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

Nature Methods · 2012 · Vol. 10(1) · pp. 57–59
Nicholas A. BokulichSathish SubramanianJeremiah J. FaithDirk GeversJeffrey I. GordonRob KnightDavid A. MillsJ. Gregory Caporaso
Microbial Community Ecology and PhysiologyGenomics and Phylogenetic StudiesGut microbiota and healthAmplicon sequencingIllumina dye sequencingComputational biologyAmpliconDeep sequencingBiologyDNA sequencingQuality (philosophy)Computer scienceGenetics

MeSH terms

HumansQuality ControlSequence Analysis, DNABiodiversityHigh-Throughput Nucleotide Sequencing
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References
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
Applied and Environmental Microbiology · 2006 · 11,173 citations
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample
Proceedings of the National Academy of Sciences · 2010 · 9,826 citations
Search and clustering orders of magnitude faster than BLAST
Bioinformatics · 2010 · 21,473 citations
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
Applied and Environmental Microbiology · 2007 · 20,269 citations
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
Applied and Environmental Microbiology · 2005 · 8,476 citations
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