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A more efficient method to generate integration-free human iPS cells

Nature Methods · 2011 · Vol. 8(5) · pp. 409–412

Keisuke Okita✉(Kyoto University)Yasuko Matsumura(Kyoto University)Yoshiko Sato(Kyoto University)A. Okada(Kyoto University)Asuka Morizane(Kyoto University)Satoshi Okamoto(RIKEN Center for Biosystems Dynamics Research)Hyenjong Hong(Kyoto University)Masato Nakagawa(Kyoto University)Koji Tanabe(Kyoto University)Kenichi Tezuka(Gifu University)Toshiyuki Shibata(Gifu University)Takahiro Kunisada(Gifu University)Masayo Takahashi(RIKEN Center for Biosystems Dynamics Research)Jun Takahashi(Kyoto University)Hiroh SajiShinya Yamanaka✉(Japan Science and Technology Agency)

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Pluripotent Stem Cells Research CRISPR and Genetic Engineering Biomedical Ethics and RegulationInduced pluripotent stem cellHuman leukocyte antigenTransgeneBiologyPlasmidPopulationComputational biologyCell biologyMolecular biologyEmbryonic stem cell

MeSH terms

Gene FrequencyGenetic VectorsHLA AntigensHumansPlasmidsTissue DonorsElectroporationCell Culture TechniquesGene Expression ProfilingAsian PeopleInduced Pluripotent Stem Cells

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An Efficient Nonviral Method to Generate Integration-Free Human-Induced Pluripotent Stem Cells from Cord Blood and Peripheral Blood Cells