articleTop 10% cited
Efficient selection for high-expression transfectants with a novel eukaryotic vector
Gene · 1991 · Vol. 108(2) · pp. 193–199
Hitoshi Niwa(Kumamoto University)Ken‐ichi Yamamura(Kumamoto University)Jun‐ichi Miyazaki✉(Kumamoto University)
RNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy researchBiologyVector (molecular biology)Selection (genetic algorithm)Computational biologyGeneticsTransfectionExpression vectorRecombinant DNACell biologyMolecular biology
MeSH terms
ActinsAnimalsbeta-GalactosidaseCattleGenetic VectorsGentamicinsCricetinaeHumansInterleukin-2L CellsPhosphotransferasesPromoter Regions, GeneticRecombinant ProteinsSelection, GeneticTransfection
Funding
- Ministry of Education, Culture, Sports, Science and Technology
Citations
5,175
FWCI
4.24
field-weighted impact
References
61
Percentile
95%
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References
High-efficiency transformation of mammalian cells by plasmid DNA.
Molecular and Cellular Biology · 1987 · 5,334 citations
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Proceedings of the National Academy of Sciences · 1987 · 4,967 citations
Establishment of mouse cell lines which constitutively secrete large quantities of interleukin 2, 3, 4 or 5, using modified cDNA expression vectors
European Journal of Immunology · 1988 · 1,091 citations
High-Efficiency Cloning of Full-Length cDNA
Molecular and Cellular Biology · 1982 · 1,278 citations
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