articleTop 1% cited

Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

Journal of Immunological Methods · 1997 · Vol. 201(1) · pp. 35–55

Anke Krebber(University of Zurich)Susanne Bornhauser(University of Zurich)Jörg Burmester(University of Zurich)Annemarie Honegger(University of Zurich)Jörg Willuda(University of Zurich)Hans Rudolf Bosshard(University of Zurich)Andreas Plückthun✉(University of Zurich)

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Monoclonal and Polyclonal Antibodies Research Glycosylation and Glycoproteins Research Protein purification and stabilityCloning (programming)Molecular biologyRecombinant DNAPhage displayBiologyGeneMolecular cloningMonoclonal antibodyImmunoglobulin light chainAntibody

MeSH terms

Amino Acid SequenceAnimalsAntibodies, MonoclonalBase SequenceBinding Sites, AntibodyCloning, MolecularGenes, ImmunoglobulinGenetic VectorsHybridomasImmunoglobulin Variable RegionMolecular Sequence DataRecombinant Fusion ProteinsSpleenStructure-Activity RelationshipPolymerase Chain Reaction

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