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By-passing immunization

Journal of Molecular Biology · 1991 · Vol. 222(3) · pp. 581–597

James D. Marks(MRC Laboratory of Molecular Biology)Hennie R. Hoogenboom(MRC Laboratory of Molecular Biology)Timothy P. Bonnert(MRC Laboratory of Molecular Biology)John McCafferty(Recombinant Antibody Technology (United Kingdom))Andrew D. Griffiths(MRC Laboratory of Molecular Biology)Greg Winter✉(MRC Laboratory of Molecular Biology)

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Monoclonal and Polyclonal Antibodies Research Glycosylation and Glycoproteins Research Bacteriophages and microbial interactionsImmunoglobulin light chainPanning (audio)HaptenAntigenPhage displayAntibodyMolecular biologyGeneBiologyPhagemid

MeSH terms

Amino Acid SequenceBacteriophagesBase SequenceBinding Sites, AntibodyDNA, Single-StrandedHumansImmunoglobulin FragmentsImmunoglobulin Variable RegionImmunoglobulin kappa-ChainsImmunoglobulin lambda-ChainsLymphocytesMolecular Sequence DataMutationSequence Homology, Nucleic AcidProtein Engineering

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Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system