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A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

The Journal of Immunology · 1979 · Vol. 123(4) · pp. 1548–1550

John F. Kearney✉(University of Cologne)Andreas Radbruch(University of Alabama at Birmingham)B Liesegang(University of Cologne)Klaus Rajewsky(University of Cologne)

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Abstract

We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.

Monoclonal and Polyclonal Antibodies Research HER2/EGFR in Cancer Research Glycosylation and Glycoproteins ResearchAntibodyImmunoglobulin light chainclone (Java method)Cell cultureCell fusionMolecular biologyMonoclonal antibodyBiologyMultiple myelomaCell

MeSH terms

AnimalsAntibody-Producing CellsCell FusionCell LineCell Transformation, NeoplasticEnzyme-Linked Immunosorbent AssayHybrid CellsImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMice, Inbred C57BLMultiple MyelomaRabbitsMice

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