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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Gene · 1989 · Vol. 77(1) · pp. 61–68
RNA and protein synthesis mechanismsGenomics and Phylogenetic StudiesMolecular Biology Techniques and ApplicationsBiologyOverlap extension polymerase chain reactionRestriction enzymeGeneticsGeneRNA splicingDNAComputational biologyPolymerase chain reactionMolecular biology

MeSH terms

Amino Acid SequenceAnimalsDNA-Directed DNA PolymeraseDNA, RecombinantExonsGenes, MHC Class IGenetic EngineeringIntronsMolecular Sequence DataRecombinant Fusion ProteinsRecombinant ProteinsTemplates, GeneticTaq PolymeraseMice
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