Neuroprotective activity of methanolic extract of leaves from Oenothera rosea in pc-12 cell line

Abstract

In this study, we evaluated the neurotoxicity and neuroprotective properties of the methanolic extract obtained from leaves of Oenothera rosea in PC-12 cell line. The PC-12 cell line was used to evaluate neurotoxicity and neuroprotection (MTT cell viability assay) and to evaluate radical scavenging activity (DCFDA assay) using different concentrations of the extract. DPPH was also used to evaluate antioxidant activity. Low neurotoxicity was observed, and all concentrations maintained a cell viability percentage above 80%. The concentration of 25 µg/mL was the most active in the neuroprotection assay, with a cell viability of 82.41±5.97, even higher than the control used for neuroprotection Trolox 50µM (76.19±2.98%). The antioxidant properties were evaluated with DCFDA and DPPH, an inhibition of free radicals was reduced in more than an 80%. Methanolic extract of leaves from O. rosea present neuroprotective activity and low cytotoxicity in vitro.