Comparison of extraction technique on recovery of phenolic compounds obtained from papaya leaves (Carica papaya L. leaves)

Abstract

This study was conducted to understand the different extraction methods viz conventional, microwave-assisted extraction and ultrasound-assisted extraction by extracting the phytochemicals from the leaves of Carica papaya L. & the influence of process parameters viz, temperature, ultrasound power and microwave power. Experiments were conducted using the single-factor experiment design wherein one factor was varied at a time keeping other factors constant. Variables selected for the experiment were Temperature (40, 50, 60 °C) & Time (30 min.) in case of conventional extraction; Power (150, 175, 200), Time (30 min) in ultrasound-assisted extraction and Power (400, 500, 600 W), Time (30 seconds) in microwave-assisted extraction. Carica papaya L. The analysis showed the TPC ranged from 25.28 mg GAE/g to 41.60 mg GAE/g, TFC ranged from 50.70 mg QE/g to 13.60 mg QE/g & 15.27% to 25.62%. The highest Total phenolic content, Total Flavonoid Content & Antioxidant activity was observed in treatment 6 employing ultrasound assisted extraction with power 200 watts. It is concluded from the experiment to extract phytochemicals from papaya leaf ultrasound can be employed.