Identification of novel compounds using chromatographic methods and screening for protease activity and anti-inflammatory activity of Adhatoda vasica

Abstract

This investigation column eluted fractions of leaf Adhatoda vasica of was assessed for its phytochemical screening, column chromatography, Thin layer chromatographic studies, protease activity, anti-inflammatory activity, antidiabetic activity and antioxidant activity. Phytochemical screening reflect the presence of alkaloid, flavonoids, coumarins, terpenoids, steroids, emodins, Quinone’s. Column chromatography method was used for purification of bioactive compounds. Thin layer chromatographic study was carried out by using various solvent system of different type of polarity of n- butanol, acetic acid and acetone. TLC profiling shows pure band at 254nm and 366 nm. The strong “proteolytic activity” also pointed out in purified fraction of eluted fraction In vitro anti-inflammatory activity was evaluated using albumin denaturation fraction 3,showinghighest activity 75% followed by fraction 5 (62.73%), membrane stabilization assay fraction 6 (80.23%) followed by fraction 3 (64.65) and proteinase inhibitory activity of fraction 5(88%) followed by fraction 787.68% at conc. 500 µg/ml. Aspirin (90.87%)was used as standard drug for the study of anti-inflammatory activity.