Identification of some constituents of Helicteres guazumifolia Kunth (Malvaceae) leaves from Sucre state, Venezuela

Abstract

Some constituents of Helicteres guazumifolia Kunth leaves extracts, which were obtained by maceration in petroleum ether and methanol, were proposed to separate and identify. Chemical separation was performed by column and thin layer chromatography, while identification was done by Gas Chromatography-Mass Spectrometric analysis (GC-MS) and 1D-2D Nuclear Magnetic Resonance experiments (1H, 13C, DEPT-135°, HMQC, HMBC, COSY 1H-1H). Several fatty constituents were isolates from non-polar fractions of this Helicteres specie, such as: methyl cis-13-docosenoate, 1-docosanol, and decyl decanoate. Other constituents were identified by GC-MS as 2-dodecanone, methyl hexadecanoate, butyl (2-methylbutyl) phthalate, ethyl hexadecanoate, n-eicosane, di (2-ethylhexyl) phthalate, (22E, 24R)-stigmasta-4,22-dien-3-one, δ-stigmast-4-en-3-one, benzophenone, (4-methylphenyl) phenylmethanone, 6,10,14-trimethylpentadeca-2-one, dibutylphtalate, 4,8,12,16-tetramethylheptadecan-4-olide, and cyclic 1,2-ethanediyl acetal (5α)-4,4-dimethyl-cholestan-3-one. Results suggest that H. guazumifolia Kunth could biosynthesize several chemical families, including fatty acid, hydrocarbon, terpenoid, steroid, and phenolic derivatives, which could suggest its possible ethnomedical uses.