Phytochemical analysis, analgesic, anti-inflammatory, antispasmodics and antioxidants activities of Chenopodium album

Abstract

In the present research work Anti-inflammatory, analgesic, antioxidant, antispasmodic activities and phytochemicals potential of Chenopodium album was carried out. The plant was extracted using (methanol, ethanol and aqueous) solvents. Acetic acid induced writhing test was used for analgesic activity. Anti-inflammatory activity was evaluated by carrageenan-induced mice paw edema. For antioxidant activity used 2, 2-diphenyl-1-picrylhydrazyl (DPPH). For antispasmodic activity charcoal was used. In qualitative investigation, the phytochemical such as carbohydrates, alkaloids, phenols, flavonoids, saponins, tannins and phlobatannins were screened. In analgesic activity after the injection of methanolic extract at a dose of 300mg/kg the writhing was reduced to (21±0.13)/5 minutes and showed 63.44% inhibition, compared with standard drug aspirin which reduced to (14±0.21), 78.43% inhibition. In anti-inflammatory activity at a dose of 300 mg/kg after four hours showed significant activity when compared with standard drug Diclofenac sodium. The maximum rate of inhibition of antioxidant was observed in the methanolic extract. In the qualitative analysis of the methanol extract showed highest amount of phenols. The atropine sulphate showed 87.34% inhibition. The extract 100mg/Kg, 200mg/Kg and 300mg/Kg showed 43.5%, 52.23%, 69.38% inhibition as compared to the standard drug atropine sulphate. We concluded that the Chenopodium album plant showed high potential of inflammation, antioxidant, antispasmodic activities and phytochemical compounds.