In vitro investigation of antioxidant potentiality of methanol and silver nanoparticles extract from Trigonella foenum-graecum

Abstract

Trigonella foenum-graecum (Fenugreek) is an important spice, its dried seeds have wide application in food and beverages as a flavoring additive as well as in medicines. Crude extracts of fenugreek were prepared by soxhelt extraction method with methanol solvent. Methanol extracts and silver nanoparticle extracts were subjected for the measurement of phytochemical assays, total phenolic content by Folin-Ciocalteu method as well as flavonoid content, chelating activity, reducing power and antioxidant/radical scavenging activity [1,1-diphenyl-2-picryl-hydrazyl. Results from these different parameters were in agreement with each other. The results revealed that the extracts of the fenugreek contains alkaloids, saponins, phytosterols, tannins, flavonoids, diterpens and exhibit antioxidant activity. These findings suggest that the fenugreek extracts could act as potent source of antioxidants. The anti-inflammatory effect of the silver nanoparticle and methanol extract of Trigonella foenum-graecum was investigated and even antimicrobial activity also was checked against six different strains of bacteria. Using these parameters antioxidant activity was assessed and the result revealed that fenugreek seed extract showed a inhibition of DPPH in lower concentrations.