Phytochemical analysis and antioxidant activity of Trigonella foenum-graecum seeds

Abstract

Trigonella foenum graecum (Fenugreek) (Family: Leguminosae) is used as a traditional medicine in treating various diseases due to its antiinflammatory, antidiabetic and antilipidemic properties. In our study, aqueous extract of seeds were subjected for in vitro antioxidant activity by 1- diphenylpicryl- hydrazyl radical (DPPH), and ABTS radical cation assay. Total phenol and total flavonoid content were estimated. It was observed that free radicals were scavenged by the extract in a dose dependent manner. The content of total phenolics (expressed as mg of gallic acid equivalents/gm extract) and total flavonoids (expressed as mg of quercetin equivalent/gm extract) were determined along with antioxidant enzymes. The aqueous extract of fenugreek exhibited potent DPPH and ABTS radical scavenging activity with IC50 values of 62.67 μg/mL and 71.44 μg/mL, respectively. Based on the results it can be concluded that aqueous seed extract of fenugreek seeds may have potential antioxidant effects against several oxidants generated in the body.