Assessment of molecular diversity in Indian mustard [Brassica juncea (L.) Czern and Coss.] using SSR markers

Abstract

Molecular diversity analysis of 38 Indian mustard genotypes was carried out employing 18 Simple Sequence Repeats (SSR) related to Resistant Gene Homologue (RGH) loci in the Department of Genetics and Plant Breeding, Dharwad, during 2018-19. These SSRs grouped the genotypes into five major clusters at similarity coefficient of 0.001, with cluster I consisted of 13 genotypes, cluster II and cluster II had six genotypes each. While cluster IV consisted of nine genotypes and cluster V with four genotypes. From 18 markers used for amplification, 130 alleles were amplified across 38 genotypes. Among them 128 alleles were scored polymorphic with 77 per cent average polymorphism. The dissimilarity value was lowest for the genotypes RH 1650 and RH 1656 which shared considerably high bootstrap value of 90.