Assessment of genetic diversity using SSR marker in moonbeam [Vigna radiata (L.) wilczek] genotypes

Abstract

Simple Sequence Repeats (SSR) markers were used to assessing of genetic diversity in 24 mungbean genotypes. Total 10 SSR markers (primers) were used, out of 10 markers nine markers shown polymorphism and only one marker shown monomorphic nature for molecular diversity. After analysis a total of 20 alleles were detected in 24 genotypes on all chromosomes. The number of alleles per locus generated by each marker ranged from 1 to 3 alleles with an average number of 2 alleles per locus. Cluster analysis was performed using Jaccard’s similarity matrices calculated from SSR markers to generate a dendogram of 24 parental lines could be grouped into eight clusters. Similarity ranged from 0.30 to 1.00 with an average similarity of 0.65 or 65% for molecular based diversity. Cluster VI had maximum eight genotypes under it followed by cluster VII had six genotypes and cluster IV had three genotypes. Cluster III and V each have two genotypes. Cluster I, II and VIII each have single genotype only. Maximum similarity of 100% was shown among genotypes CO 6 and RM 03-71 in cluster VI; Pairy Mung, Malviya Jyoti & Pratiksha and Pusa Vishal & HUM -12 in cluster VII.