Assessment of the molecular diversity of a set of wheat genotypes

Abstract

The genetic diversity of a set of hexaploid and tetraploid wheat genotypes at biotechnology centre Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola was investigated by applying 21 simple sequence repeats (SSR) primers and only six primers gave ploymorphism. The materials consisted of eleven bread wheat and five durum wheat. In total 93 alleles were detected with an average of 15.5 alleles per locus. The average PIC values per locus varied from 0.77 for the primer Xgwm-136 to 0.92 for the primer Xgwm-130. Genetic similarity values between genotypes, calculated by Jaccard’s similarity coefficient, were used to produce a dendrogram. In the dendrogram the genotypes were clustered in three main clusters and two genotypes remained solitary in dendrogram.