ISSR studies on small and large seed varieties of Glycine max

Abstract

Plant molecular identity (ID) is required to describe molecular characteristics of plants, which is should contain all of the required information. Molecular markers are popularly used to characterize plant genetic diversity. In the present study, DNA was isolated from the two different Glycine max varieties, for ISSR based marker analysis. For molecular characterization total 30 primers of ISSR were tested for the both varieties. The results obtained using ISSR primers for two Glycine max varieties showed recordable monomorphic and polymorphic band patterns. Overall, ISSR has shown 39.2 % polymorphism. In this, primers UBC888, UBC889 and UBC814 showed 100% polymorphism, followed by 73.33% in UBC881 and 77.78% in UBC817. Thus primers UBC888, UBC889 and UBC814, have given high polymorphism and proved the best to distinguish both varieties from each other.