Molecular diversity in rice (Oryza Sativa L.) accessions of Chhattisgarh based on grain length using ISSR markers

Abstract

A total of ten ISSR primers were taken to assess the genetic diversity. The 10 primers yielded a total of 46 amplified fragments from 48 rice genotypes and out of these, 29 alleles were polymorphic. The number of scorable bands produced per primer ranged from 2 to 6 with an average of 4.6, and the average number of polymorphic fragments per primer was 2.9. The highest number of alleles (6) was detected on each of locus UBC 809, UBC 834, UBC 841, UBC 842, UBC 873 and the lowest number of alleles (2) was detected on locus UBC 824. The polymorphism percentage ranged from 33.33% (primer UBC 834) to 100% (primer UBC 824, UBC 841, UBC 856) with an average polymorphism of 60% across all the genotypes. The UPGMA based clustering analysis using similarity coefficient grouped these genotypes into two major and three sub-clusters. Cluster I and II consists of 24 genotypes. The grouping resembled the ancestry of the genotypes under study.