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Rapid isolation of high molecular weight plant DNA
Nucleic Acids Research · 1980 · Vol. 8(19) · pp. 4321–4326
Michael G. Murray✉(Carnegie Institution for Science)William F. Thompson(Carnegie Institution for Science)
Abstract
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.
Chromosomal and Genetic VariationsPlant tissue culture and regenerationPlant Genetic and Mutation StudiesBiologyDNARestriction enzymeIsolation (microbiology)Mitochondrial DNAChloroplast DNANuclear DNADigestion (alchemy)Molecular biologyGenetics
MeSH terms
DNAIndicators and ReagentsMethodsMolecular WeightPlants
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