HPTLC and GC-MS analysis of Parthenocissus renukae Anto & Pradeep

Abstract

Parthenocissus renukae Anto & Pradeep belongs to family Vitaceae is a woody climber that spreads on rocks, grasslands and climb on large trees. It may badly affect the supporting tree by completely covering its canopy finally leading to the death of host tree. The study aims to find out the presence of alkaloid by HPTLC analysis with alkaloid as standard reference marker and to identify the secondary metabolites and bioactive compounds from the stem ethanol extract of P. renukae, by Gas chromatography and Mass spectroscopy (GC-MS). HPTLC analysis shows similar retention factor values and peaks between sample and alkaloid reference marker that confirms the presence of alkaloid. GC-MS shows the presence of alkaloids, fatty acid esters, flavonoids and alcohols and also other bioactive compounds like are 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Hexadecanoic acid, Hexadecanoic acid ethyl ester, Octadecanoic acid ethyl ester and Docosenamide. Further studies are needed to isolate active compounds of the extract as well as to reveal their exact mechanism of action in various diseases.