Spectral analysis of methanol extract of Cissus quadrangularis L. stem and its fractions

Abstract

The Objective: The stem of Cissus quadrangularis L. (Vitaceae) is reported to have great medicinal value. The aim of the present study was to determine the chemical profile of methanol extract of Cissus quadrangularis and its fractions (by various spectral analysis) which will be useful for its proper identification when therapeutically used. Methods: Spectral analysis was done by different analytical methods such as UV, IR, GC-MS and HPTLC studies in methanol extract (ME) of Cissus quadrangularis and its fractions (FS-I and FS-II). Results: In HPTLC analysis, ME and its fractions showed the presence of different types of compound with different Rf values ranging from 0.01 to 1.05. Maximum number of compounds were in FS-I (24). The GC-MS analysis showed the presence of fatty acids such as hexadecanoic acid (CAS) palmitic acid, octadecanoic acid (CAS) stearic acid, 9, 12-octadecadienoic acid (Z, Z)-(CAS) linoleic acid, 2-hexadecenoic acid, methyl ester 7, 10-Octadecadienoic acid methyl ester, linoleic acid, butyl ester and 9, 12-octadecadienoic acid (Z, Z)-, 2, 3-dihydroxypropyl ester in ME and its fractions of Cissus quadrangularis L. Conclusions: Spectral analysis is useful in differentiating the species from the adulterants and the results of this study may act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.