Evaluation of antioxidant, antibacterial, alpha amylase enzyme inhibition activities and GC-MS analysis of leaves extract of Gymnema sylvestre L.

Abstract

Gymnema sylvestre is an important medicinal plant which belongs to the family Apocynaceae, commonly used herb in Ayurveda. The common name is gurmar, meaning of “sugar destroying,” was given because of the plant has antisacharogenic property (suppresses the taste of sugar). The aim of the present study was to evaluate the antioxidant, antibacterial activities and to identify the bioactive compounds by GC-MS analysis of methanol leaves extract of G. sylvestre. Different antioxidant assays were carried out for evaluating antioxidant activity. The maximum DPPH radical scavenging activity was 54.08±0.15% at 120 µg/mL concentration with the IC50 of 104.66 µg/mL concentration. The maximum DPPH radical scavenging activity was 54.08±0.15% at 120 µg/mL concentration with the IC50 of 104.66 µg/mL concentration. The maximum superoxide radical (O2·-) scavenging activity was 62.48±0.20% at 120 µg/mL concentration with the IC50 of 72.93 µg/mL concentration. The maximum Fe3+ reduction and Mo6+ reduction were 83.11±0.17% and 62.03±0.36% at 120 µg/mL concentration with the RC50 of 43.79 µg/mL and 75.14 µg/mL concentration respectively. The maximum α-amylase enzyme inhibition was 70.86±0.94% at 60 µg/mL concentration with the IC50 of 75.40 µg/mL concentration.