Identification and quantification of squalene in Europaean spindle seed oil (Euonymus europaeus L.) by optimized high performance liquid chromatography (HPLC) procedure

Abstract

In Europaean spindle seed oil (Euonymus europaeus L.) was identified squalene. The oil from the seeds was extracted with hexane. The procedureby reverse phase high-performance liquid chromatography with UV detection (RP-HPLC UV) was optimizedfor identification and quantification.Chromatographic separation was carried out in isocratic mode, a mobile phase of acetonitrile: tetrahydrofuran (90:10) on a column of Luna® Omega Polar C18 100 A, 250 mm × 4.6 mm, 5 μm at 30 °C. The retention time of squalene in the test sample was 9 minutes. The content of squalene in the studied oil was 1.84 mg / ml ± 0.05 mg / ml. The high content of squalene in combination with carotenoids, tocopherols and unsaturated fatty acids can be the cause of the effectiveness of spindle tree oil in the treatment of dermatological diseases.