Identification and quantification flavonoids in two wild edible plants, Viburnum foetidum and Perilla ocimoides of North-Eastern region in India, using high performance liquid chromatography with diode array detection

Abstract

Identification and quantification of flavonoids (aesculin, catechin, rutin, naringin, myrecetin, coumarin, luteolin, quercetin, naringenin, apigenin and kaempferol) in two different solvent extracts (methanol and 80% aq. ethanol) of two wild edible leaves of viz. Viburnum foetidum and Perilla ocimoides, collected from North-eastern region in India has been performed using a reversed-phase high-performance liquid chromatography with photodiode array detector. The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm), Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272, 280 and 310 nm) using a mobile phase of acetonitrile and water with gradient elution. The experimental results showed high amount of myricetin (36.19±0.020 mg/gm dry extract) and luteolin (30.242±0.033 mg/gm dry extract) present in the 80% aq. ethanol extract of P. ocimoides and a good amount of naringin (18.304±0.022 mg/gm dry extract) is detected in the methanol extract of V. foetidum. The high percentage of recovery (97-100%), low coefficient of variation (R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of flavonoid compounds in the two plants under investigation.