Phytochemical screening and characterization of bioactive compounds in Kalarchi chooranam using GC-MS technique

Abstract

The aim of this study was to carry out for identification of bioactive compounds from the Kalarchi chooranam by qualitatively and Gas chromatography-Mass Spectroscopy (GC-MS) technique. The qualitative analysis of Kalarchi chooranam showed the presence of alkaloids, phenols, saponins and phytosterols while protein, aminoacids and flavonoids were absent. GCMS analysis of Kalarchi chooranam was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry while the mass spectra of the compounds found in the Kalarchi chooranam was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of fifteen compounds. The prevailing compounds are 2,5-Furandione, dihydro-3-methylene, Phthalic anhydride and L-Glutamic acid 5-ethyl ester. These findings support the traditional use of Kalarchi chooranam in various disorders.