Determination of bioactive compounds in Ziziphus oenoplia leaves extract using gas chromatography and mass spectroscopic technique

Abstract

Phytochemicals are the chemicals extracted from plants. These organic chemicals are classified as primary or secondary constituents, depending on their role in plant metabolism. GC-MS method used for the analysis of the obtained extract can be an interesting tool for testing the amount of some active principles in herbs used in various industries. The aim of this study was to carry out for identification of bioactive compounds from ethanolic extract of Ziziphus oenoplia leaves by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of ethanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis of Ziziphus oenoplia leaves extract revealed the presence of various compounds like Heptadecane, Hexadecanoic acid, ethyl ester, Pentadecanoic acid, 2-Hexadecen-1-ol, 3,7,11,15-Tetramethyl, 1,2-Benzenedicarboxylic acid, dihexyl ester and 9-Octadecenoic acid. These findings support the traditional use of Ziziphus oenoplia leaves in various disorders.